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nfκb luc cho k1 cell line  (BPS Bioscience)


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    Structured Review

    BPS Bioscience nfκb luc cho k1 cell line
    Luciferase activities in <t>NFκB-Luc/CHO-K1</t> cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, <t>NFκB</t> <t>luciferase</t> was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.
    Nfκb Luc Cho K1 Cell Line, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nfκb luc cho k1 cell line/product/BPS Bioscience
    Average 92 stars, based on 1 article reviews
    nfκb luc cho k1 cell line - by Bioz Stars, 2026-02
    92/100 stars

    Images

    1) Product Images from "The Cholesterol Metabolite Cholest-5-en-3-One Alleviates Hyperglycemia and Hyperinsulinemia in Obese ( db / db ) Mice"

    Article Title: The Cholesterol Metabolite Cholest-5-en-3-One Alleviates Hyperglycemia and Hyperinsulinemia in Obese ( db / db ) Mice

    Journal: Metabolites

    doi: 10.3390/metabo12010026

    Luciferase activities in NFκB-Luc/CHO-K1 cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, NFκB luciferase was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.
    Figure Legend Snippet: Luciferase activities in NFκB-Luc/CHO-K1 cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, NFκB luciferase was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.

    Techniques Used: Luciferase, Incubation



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    BPS Bioscience nfκb luc cho k1 cell line
    Luciferase activities in <t>NFκB-Luc/CHO-K1</t> cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, <t>NFκB</t> <t>luciferase</t> was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.
    Nfκb Luc Cho K1 Cell Line, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nfκb luc cho k1 cell line/product/BPS Bioscience
    Average 92 stars, based on 1 article reviews
    nfκb luc cho k1 cell line - by Bioz Stars, 2026-02
    92/100 stars
      Buy from Supplier

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    Luciferase activities in NFκB-Luc/CHO-K1 cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, NFκB luciferase was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.

    Journal: Metabolites

    Article Title: The Cholesterol Metabolite Cholest-5-en-3-One Alleviates Hyperglycemia and Hyperinsulinemia in Obese ( db / db ) Mice

    doi: 10.3390/metabo12010026

    Figure Lengend Snippet: Luciferase activities in NFκB-Luc/CHO-K1 cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, NFκB luciferase was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.

    Article Snippet: In the NFκB-Luc/CHO-K1 cell line (BPS Bioscience, San Diego, CA, USA), fLUC expression is controlled by the NFκB response element located upstream of the TATA promoter and is suitable for monitoring the activity of the NFκB transcription factor through luminescence readout.

    Techniques: Luciferase, Incubation